Figure 5.

Microporation of human adipose tissue–derived stem cells (hADSCs) does not affect osteoblastic differentiation. Furthermore, hADSCs were transfected with the reporter plasmid, pCMV-EGFP-N1. After 24 hours, cells were cultured in control medium or osteogenic medium for 14 days. (a) Following fixation, the cells were stained by Alizarin red as described in Materials and Methods. (b) Representative high magnification fields of Alizarin red staining confirmed mineralized nodule formation in the osteo-induction group. (c) Evaluation of enhanced green fluorescent protein (EGFP) expression in microporated hADSCs. EGFP expression was detected by fluorescence microscopy. Images were taken using ×200 objectives in b and c.