Figure 6.

Microporation of human adipose tissue–derived stem cells (hADSCs) does not affect their potential for adipogenic differentiation, and hADSCs were transfected with reporter plasmid, pCMV-EGFP-N1. After 24 hours, the cells were cultured in control medium (upper row) or adipogenic medium (lower row) for 12 days. Following fixation, the cells were stained with Oil-red as described in Materials and Methods. High magnification fields of Oil-red staining confirmed oil body formation in the adipo-induction group. Enhanced green fluorescent protein (EGFP) expression was detected by fluorescence microscopy. Images were taken using ×200 objectives.