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RNA interference-induced STIM1 silencing prevented NFAT nuclear translocation and activity. (a,b) Measurement of NFAT activity using a NFAT-driven luciferase construct in control cells as in a or cells treated with thapsigargin (1 µmol/l for 6 hours) as in b (P < 0.001). (c) Localization of NFAT protein by immunofluorescence. (d) Measurement of the relative MCIP mRNA level normalized to RPL32 mRNA. Cyclosporin A (CsA) is used as a negative control (*P < 0.05).
