Figure 3.

Treatment with F3.CD cells and 5-fluorocytosine (5-FC) has a significant in vivo therapeutic effect. (a) Timeline for the metastatic brain tumor animal model and subsequent treatment using F3.CD cells and 5-FC. (b) F3.CD cells were injected into the brain contralateral to the tumor-bearing hemisphere and localized using an anti-CD antiserum. Closed arrow heads indicated cytosine deaminase (CD)-immunoreactive F3.CD cells in the corpus callosum (upper panel) and the inset shows a magnified area. (c) Compared with the other groups, mice treated with F3.CD and 5-FC (group 4) showed significantly small tumor volumes (P < 0.05). Columns = mean, bars = SE. HBSS, Hanks' balanced salt solution; i.p., intraperitoneal.