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. 2008 Dec 16;17(3):472–479. doi: 10.1038/mt.2008.272

Figure 3.

Figure 3

Thymocytes transduced by in situ injection of a scAAV2/8 vector differentiate and migrate to the periphery. (a) Expression of the enhanced green fluorescent protein (eGFP) transgene was monitored in the lymph nodes (LNs) and spleens of mice intrathymically injected with the scAAV2/8 vector. Expression was assessed by quantitative reverse transcription–PCR at days 3, 10, and 30, and was normalized to hypoxanthine-guanine phosphoribosyl transferase. Normalized means ± SD of arbitrary units (AU) are shown for duplicate samples. (b) The percentages of LN and spleen cells expressing the eGFP transgene were also monitored by flow cytometry. The means ± SD of three animals are shown for each time point. (c) Following intrathymic scAAV2/8 administration, the phenotypes of transduced lymphocytes was monitored in LNs by assessing eGFP expression in CD3 and CD19 populations as compared to mock-transduced mice (phosphate-buffered saline) (upper panels). The expression of the eGFP transgene within the CD3 subset was further evaluated by CD4 and CD8 staining. The relative ratio of CD4 and CD8 cells within the nontransduced (eGFP−) and transduced (eGFP+) fractions are shown in representative dot plots (bottom panels). scAAV, self-complementary adeno-associated virus.