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. 2008 Dec 16;17(3):463–471. doi: 10.1038/mt.2008.269

Figure 2.

Figure 2

Analysis of enhanced green fluorescent protein (EGFP) expression 2 weeks after intravitreal delivery of equal doses of wild-type (WT) scAAV8-CBA-EGFP or its tyrosine mutants. (a–c) Immunohistochemistry for EGFP in flat-mount retinas infected with WT (a) AAV8 vector, (b) mutant Y733F, or (c) mutant Y447F. Calibration bar 100 µm. All pictures were taken with the same exposure time to evaluate EGFP intensity with ImageJ. (d) Values indicate percentage of EGFP intensity of the mutants compared with WT. Only tyrosine-mutant Y733F showed a statistically significant elevation in EGFP intensity (*P < 0.0001) compared with WT AAV8. Statistical analyses were performed with one-way ANOVA plus Dunnett's multiple-range test compared to the control group (WT scAAV8). CBA, chicken β-actin; scAAV, self- complementary adeno-associated virus.