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. 2008 Dec 16;17(3):463–471. doi: 10.1038/mt.2008.269

Figure 4.

Figure 4

Comparison of enhanced green fluorescent protein (EGFP) intensity of all serotypes and their mutants with wild-type self-complementary adeno-associated virus 2 (WT scAAV2) vector 2 weeks after intravitreal delivery. Retinal flat mounts were immunolabeled with polyclonal EGFP antibody. Pictures were taken with the same exposure time to evaluate the intensity, with ImageJ. Values indicate percentage of EGFP intensity compared with wild-type AAV2 vector. Type 2 Y444F showed the highest GFP expression enhancement (***P < 0.001); type 2 mutant Y730F and type 8 Y733F also significantly EGFP expression (**P < 0.01); type 9 mutant Y446F also had a higher level of EGFP (*P < 0.05). Statistical analyses were performed with one-way ANOVA plus Dunnett's multiple-range test compared to the control group (WT scAAV2).