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. 2008 Dec 16;17(3):463–471. doi: 10.1038/mt.2008.269

Figure 6.

Figure 6

Fluorescence microscopic evaluation of enhanced green fluorescent protein (EGFP) expression in transverse sections of retinal tissue 2 weeks after intravitreal injection. Immunostaining for EGFP in sections of the retina after delivery of (a) wild-type self-complementary adeno-associated virus 2 (WT scAAV2), (b) serotype 2 tyrosine-mutant Y444F, and (c) serotype 2 tyrosine-mutant Y730F. Note intense EGFP staining throughout all retinal layers with Y444F mutant and predominant EGFP staining in the GCL with WT-2 and Y730F. Calibration bar 100 µm. gcl, ganglion cell layer; ipl, inner plexiform layer; inl, inner nuclear layer; onl, outer nuclear; os, outer segment.