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. 2008 Dec 16;17(3):463–471. doi: 10.1038/mt.2008.269

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Copyright 2009, The American Society of Gene Therapy

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Analysis of enhanced green fluorescent protein (EGFP) expression in frozen retinal sections by immunohistochemistry at 1 month following subretinal injections with highly efficient Tyr/Phe mutant adeno-associated virus vectors. Representative sections depicting widespread and intense EGFP fluorescence throughout the retina after transduction with (a) serotype 2 Y444F or (b) serotype 8 Y733F. The images are oriented with the vitreous toward the bottom and the photoreceptor layer toward the top. Calibration bar 500 µm. (c) EGFP fluorescence in photoreceptors, retinal pigment epithelial (RPE), and ganglion cells from mouse eyes injected subretinally with serotype 2 Y444F; (d) EGFP fluorescence in photoreceptors, RPE, and Müller cells after serotype 8 Y733F delivery. (e) Detection of Müller cells processes (red) by immunostaining with a glutamine synthetase (GS) antibody. (f) Merged image showing colocalization of EGFP fluorescence (green) and GS staining (red) in retinal sections from eyes treated with serotype 8 Y733F. Calibration bar 100 µm. gcl, ganglion cell layer; ipl, inner plexiform layer; inl, inner nuclear layer; onl, outer nuclear; os, outer segment.