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. 1997 Dec 9;94(25):13594–13599. doi: 10.1073/pnas.94.25.13594

Figure 1.

Figure 1

Northern blot analysis of ALDH3 expression in mouse, bovine, and human tissues. (A) Hybridization of a mouse ALDH3 cDNA probe to a Northern blot of total RNA from adult mouse cornea (2 μg), lens (20 μg), adrenal gland (20 μg), urinary bladder (20 μg), brain (20 μg), liver (20 μg), lung (20 μg), and stomach (20 μg) revealed ALDH3 transcript levels in the cornea to be at least 500-fold higher than those observed in any other tissue examined. (B) Significant levels of ALDH3 transcript also were detected in total RNA from bovine cornea (10 μg) and human corneal epithelium (10 μg) but not in human corneal endothelium (5 μg). (C) A Northern blot analysis in which the autoradiogram purposely has been overdeveloped to demonstrate hybridization of a mouse ALDH3 cDNA probe to total RNA from mouse cornea (2 μg), stomach (20 μg), urinary bladder (20 μg), lung (20 μg), and lens (20 μg), indicating very low but detectable levels of ALDH3 expression in these tissues.