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. 1997 Dec 9;94(25):13594–13599. doi: 10.1073/pnas.94.25.13594

Figure 2.

Figure 2

Histochemical examination of ALDH3 and cat reporter gene expression in the mouse cornea. (A) The structure of the adult mouse cornea is seen in a hematoxylin/eosin-stained section. The stratified corneal epithelium (epi) overlies a stromal layer (str) maintained by numerous corneal keratocytes (arrow). The one-cell-thick endothelium (endo) forms the posterior extent of the cornea. (B) Histochemical localization demonstrates ALDH3 activity primarily in the cells of the central epithelium of the normal mouse cornea. (C) Histochemical localization of CAT activity in the cornea of a −1050INT transgenic mouse demonstrates the ability of the mouse ALDH3 promoter fragment to target reporter gene expression to the corneal epithelium, faithfully reproducing the epithelial-specific pattern demonstrated for the endogenous mouse ALDH3 gene. In the peripheral cornea, localization of both ALDH3 (D) and CAT (E) activities diminishes near the limbal border (arrows), with little activity detected in limbal epithelial cells themselves.