Figure 4.

ALDH3 promoter-driven reporter gene expression in the tissues of adult transgenic mice. (A) In Xb12/cat transgenic animals (n = 4), significant expression of the reporter gene, expressed as CAT activity, is seen in stomach, bladder, cornea, lens, lung, and spleen (trace) although not at relative levels consistent with expression of the endogenous ALDH3 gene (see Fig. 1 A and C). (B) By contrast, transgene expression in −1050INT/cat transgenic mice is restricted primarily to the corneas of mice from three independent transgenic lines (n = 4 animals/transgenic line). Very low levels of transgene expression are seen in the livers of animals from each transgenic line, with CAT activity ranging from 5 to 12% of corneal activity in the respective transgenic line.