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. 2009 Feb 17;17(4):614–622. doi: 10.1038/mt.2009.9

Figure 2.

Figure 2

Cytolytic effect of viruses. (a) Cells with normal or constitutively activated HIF pathways (VHL53 and RCC-10, respectively) were infected with Ad-DHLuc, Ad-HLuc, Ad-WT, or Ad9xHRE1A viruses and maintained for 7 days under normoxic conditions until viability was determined. The percentage of surviving cells versus noninfected controls is represented. (b–d) Influence of the E1A CR2 deletion. (b) HuH-7, (c) H2T, and (d) IMR90 cells were infected with Ad-DHLuc or Ad-HLuc at the indicated MOIs and cultured under normoxia (Nx, black symbols) or hypoxia (Hx, white symbols) for 5 (HuH-7, H2T) or 9 days (IMR-90). (e) Cytolytic effect of the Ad-WT on HuH-7, H2T, and IMR-90 cells under normoxic conditions. (f) Cytolytic effect of Ad-DHLuc on human pancreatic cancer cell lines BxPC-3, AsPC-1, NP18, and PANC-1 under normoxic conditions. Viability was determined 5 days after infection in e and f panels. The percentage of surviving cells versus noninfected controls is represented as a sigmoidal dose–response curve. Each curve is a representative experiment from at least four performed in triplicate.