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. 2009 Feb 17;17(4):667–674. doi: 10.1038/mt.2009.1

Figure 3.

Figure 3

Reverse transcription kinetic assay for vector cDNA in target cells transduced with the parental and cHS4-insulated lentiviral vectors. 293T cells were transduced with equal amounts (80 ng of p24 Ag) of each vector stock, and DNA, including low-molecular-weight DNA, was subsequently extracted at various time points. (a) The total amount of the reverse-transcribed vector genome was measured in triplicate using a primer-probe set targeted at the human immunodeficiency virus 1 (HIV1) packaging signal sequence. Reverse-transcribed vector genome copy numbers of cells transduced with INS1(+) were significantly lower (P < 0.05), compared to cells transduced with the parental vector at 6, 12, 50, and 144 hours after transduction. (b) 2-LTR circle formation was measured using quantitative PCR. (c) The peak 2-LTR circle formation at 50 hours after transduction. All experiments were performed in triplicate. *P = 0.01. LTR, long-terminal repeat.