Figure 4.

Film autoradiograms showing the spatial expression of luciferase mRNA in brain 2 weeks following an intrastriatal injection of compacted pUL3 nanoparticles. Dotted lines indicate the outline of the coronal brain section. Brain section hybridized with the antisense cRNA riboprobe (a,c) and adjacent brain section hybridized with the sense cRNA riboprobe (b). (c) Emulsion autoradiogram showing the cellular expression of luciferase mRNA (clustered dark grains) in striatal cells (gray; Nissl-stained); labeling is most likely occurring in neurons as well as glia. Black arrows point to cells showing cRNA riboprobe hybridization. a, scale bar = 1 mm; c, scale bar = 20 µm.