Figure 5.

Distribution and survival of the transplanted MSCs. (a) Hematoxylin and Eosin staining. (b) Immunohistochemical staining. The transduced and mock-transduced rat MSCs were prelabeled with bromodeoxyuridine (BrdU) and co-injected with carbon black into the quadriceps of LEW/SsNNarl rats. The location of the transplanted cells was identified with the aid of the carbon black, and the tissue specimens were removed at 1, 5, or 10 days post-transplantation. The prelabeled MSCs were probed with the anti-BrdU monoclonal antibody and visualized with the Alexa 488-conjugated secondary antibody (green), while all nucleated cells were stained by 4′,6-diamidino-2-phenylindole (red), therefore the transplanted cells appear orange after superimposing the images. n = 3 for each time point. The original magnifications were ×200 (scale bars =100 µm). The enlarged images (magnification, ×1,000) are shown in the insets. MSCs, mesenchymal stem cells.