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. 2009 Feb 24;17(5):857–863. doi: 10.1038/mt.2009.25

Figure 2.

Figure 2

AAV dose and FIX antigen level-dependent induction of immune tolerance to hFIX in FIXKO mice. Eight- to 10-week-old FIXKO mice on C57BL/6 background were given intramuscular injection of either 2 × 1010 vg (n = 7) or 1 × 1010 vg (n = 5) of AAV1–hFIX. hFIX antigen and anti-FIX IgG antibody levels were analyzed every 4 weeks after AAV injection. Results are the mean ± SEM for each time point. Closed inverted triangle: 2 × 1010 vg AAV1–hFIX, closed diamond: 1 × 1010 vg AAV1–hFIX. (a) hFIX antigen. Difference of hFIX antigen level between the two cohorts is significant (P = 0.0017 for comparison of the samples collected at the time point of 12 week after AAV injection). (b) Anti-hFIX IgG antibody. Difference of the level of anti-FIX antibodies between two cohorts is significant (P = 0.0015 for comparison of the samples collected at the time point of 12 week after AAV injection). AAV, adeno-associated virus; AAV1, AAV serotype one; FIX, coagulation factor IX; FIXKO, FIX knock out; hFIX, human FIX.