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. 2003 Nov 13;100(24):13875–13880. doi: 10.1073/pnas.2436143100

Fig. 4.

Fig. 4.

Activity stain of separated EC-SOD monomers. The EC-SOD monomers were reconstituted with Cu(II) and Zn(II) and separated by SDS/PAGE. Three micrograms of purified EC-SOD was loaded in lane 1. Approximately 1 μg of recharged monomeric EC-SOD collected in fractions 1.1 and 1.2 (Fig. 2 A) was loaded in lanes 2 and 3, respectively. The arrow indicates the position of both monomeric forms on SDS/PAGE analysis of unboiled EC-SOD. No SOD activity can be detected in lane 3. The specific activity determined by the xanthine oxidase/cytochrome c assay is shown below the gel.