Skip to main content
. Author manuscript; available in PMC: 2010 Mar 9.
Published in final edited form as: Cell Signal. 2005 May 31;18(1):93–104. doi: 10.1016/j.cellsig.2005.03.028

Fig. 5.

Fig. 5

CEP-11004 causes cell cycle arrest in pro-metaphase. HeLa cells grown on coverslips were synchronized at the G1/S boundary with excess thymidine and released back into the cell cycle. At 5 h after release, cells were treated in the absence or presence of CEP-11004 (500 nM) for an additional 4 or 6 h (9 or 11 h cumulative time after G1/S release). (A) The percentage of cells in prophase (P), pro-metaphase (PM), metaphase (M), or anaphase/telophase (AT) was determined by DAPI staining of chromosomes in control (open bars) or CEP-11004 treated (closed bars) cells. The left and right graphs represent the percentage of mitotic cells 9 and 11 h, respectively, after G1/S-phase release. Data represent the average and standard error from 3 independent experiments. (B) The number of cells in pro-metaphase were counted at 11 h after G1/S-phase release following treatment with increasing concentrations of CEP-11004 (added at 5 h after G1/S-phase release). 300–350 cells were counted at each concentration.