Figure 3.

CTL assays. (a) In vitro augmentation of the cytolytic activities of the spleen. After pmPSCA immunization, the specific cytotoxicity was greatest at an effector target ratio of 20:1. The data shown represents one of two separate experiments with similar results. (b) Adoptive transfer of the lymphocytes. Mice (n = 6) received s.c., injections of a mixture of TRAMPC1 cells and splenocytes either from cured mice or naive mice. All mice receiving splenocytes from pmPSCA vaccinated cured mice failed to grow tumors, resulting in survival >100 days whereas tumors developed in all animals receiving splenocytes from naive mice. CTL, cytotoxic T lymphocyte assays; s.c., subcutaneous.