Figure 5.

Detection of hMADS cells–derived nuclei in mouse muscles. The presence of hMADS cells–derived nuclei was assessed 1 month after injection of hMADS cells in the tibialis anterior muscles of Rag2^−/− γC^−/− mice. hMADS cells–derived nuclei were labeled on transverse cryostat sections with a human specific anti-lamin A/C antibody conjugated to Alexa Fluor 488 (green). The basal lamina was labeled with an antibody crossreacting with both human and mouse laminin and detected with Alexa Fluor 405 (blue). Human positive nuclei were found as central nuclei in regenerated fibers (arrow) or in cells located in interstitial spaces (arrowhead). Nuclei derived from MyoD-hMADS cells are presented. The same immunofluorescent pattern was observed for the different types of hMADS cells, except that MyoD-hMADS cell–derived nuclei were more often observed integrated in the muscle fibers than nuclei derived from LacZ- or WT-hMADS cells. Bar = 10 µm. hMADS, human multipotent adipose-derived stem; WT, wild type.