Figure 1.

Generation and characterization of the recombinant viruses. (a) Schematic representation of the parental and recombinant measles virus full-length cDNA genomes. Echistatin and cyclic RGD cDNAs were inserted at the COOH-terminal of measles hemagglutinin (H) protein as SfiI–NotI inserts. (b) Immunoblot of the parental and chimeric virions using anti-H and antinucleocapsid (N) antibodies. Equal titers of each virus were loaded. Lane 1, MV-GEcs; Lane 2, MV-GcRGD; Lane 3, MV-GFP. (c) RNA extracted from infected Vero cells were analyzed by RT-PCR to detect the respective H transcripts. (d,e) One-step growth curves of recombinant viruses in Vero producer cells. Cells were infected at MOI of 3.0 TCID₅₀/cell and cell-associated (d) and released virus (e) were harvested and titrated on Vero cells at intervals following infection. cDNA, complementary DNA; MOI, multiplicity of infection; RGD, arginine-glycine-aspartate; RT-PCR, reverse transcription-PCR.