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. 2009 Feb 24;17(6):1003–1011. doi: 10.1038/mt.2009.22

Figure 4.

Figure 4

Selective cytotoxic effects of HMGB2 promoter-driven suicide gene expression. (a) Cellular effects as visualized under a microscope. Cells were transduced for 24 hours with either BV-HMGB2-TK or BV-Promoterless-TK, at an MOI 10 for astrocytes and U87 cells and at an MOI 100 for neurons, followed by treatment with the prodrug ganciclovir GCV (50 or 500 µmol/l) for another 24 hours. (b) Cell viability analysis of BV-HMGB2-TK transduced U87 glioblastoma cells and normal human astrocytes (NHAs) using quantitative MTS assays. Cells were transduced in the same way as above, followed by treatment with the prodrug ganciclovir GCV (50, 100, 250, or 500 µmol/l) for another 24 or 48 hours. Results are expressed as % BV-CMV-eGFP transduced cells. *P < 0.05, **P < 0.01, ***P < 0.001 vs. BV-Promoterless-TK category treated at the same contraction of GCV by ANOVA. ANOVA, analysis of variance; HMGB2, human high mobility group box2; GCV, ganciclovir; NHA, normal human astrocyte.