Figure 2.
Histological assessment of respiratory epithelial damage in the airways following infection with Pseudomonas aeruginosa. (a) Hematoxylin and eosin-stained lung section from control mice and (b,c) hematoxylin and eosin-stained lung sections from P. aeruginosa-infected mice. Bacteria injected at a dose of 105 cfu induced only local damage to the respiratory epithelium (b). Evidence of extensive damage can be observed on lung sections from mice inoculated with P. aeruginosa at a dose of 106 cfu (c). The mice were killed 48 hours postinstillation. Micrographs are representative of lung sections obtained from three animals per group. Staining was performed on 5–10 sections per lobe. Original Magnification ×20. cfu, colony forming units.