Figure 4.

Serum amino acids and alloisoleucine in iMSUD–HTx mice suggest catabolism at killing. Blood was collected by cardiac puncture at killing and the serum analyzed by ion-exchange HPLC for (a) BCAAs (valine, isoleucine, and leucine), (b) alloisoleucine, (c) glycine, and (d) alanine. Results are represented as the mean ± SEM (*P < 0.01 compared to controls; **P < 0.001 compared to controls). The number displayed below each bar indicates the n value. BCAA, branched-chain amino acids; HPLC, high-performance liquid chromatography; HTx, hepatocyte transplantation; iMSUD, intermediate maple syrup urine disease.