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. 2009 Mar 3;17(7):1145–1154. doi: 10.1038/mt.2009.35

Figure 3.

Figure 3

FVIII expression from lentivirally transduced human hematopoietic cells lines. Cell lines K562 (myeloid-erythroid CML), EU-1 (B cell ALL), U.937 (monocytic lymphoma) and Jurkat (acute T-cell lymphoma) were transduced with the indicated fVIII transgene and assayed for clotting activity and transcript copy number. (a) FVIII activity was measured by one-stage coagulation assay of conditioned medium from cells transduced at an MOI = 1 with HIV-HP-fVIII. Each bar represents the mean activity of two wells. (b) Clotting activity was normalized to copy number (dark bars) or transcript number (gray bars) as determined by quantitative RT-PCR. (c) A schematic diagram of SIV-based-HP-fVIII, which was used for the transductions presented in d and e. (d) FVIII activity was measured by one-stage coagulation assay of conditioned medium from cells transduced at an MOI = 10 with SIV-HP-fVIII. Each bar represents the mean activity of two wells. (e) Clotting activity was normalized to proviral copy number (dark bars) or transcript number (gray bars) as determined by quantitative RT-PCR.