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Time-dependent changes in gene expression after intratracheal administration of PEG-b-P[Asp(DET)] polyplex nanomicelle loaded with luciferase gene. The mice (five mice per group) were anesthetized and the polyplex nanomicelle was administered intratracheally. After the indicated time, the lung tissues were harvested, homogenized, and measured for luciferase activity (mean ± SEM, N = 5). PEG-b-P[Asp(DET)], PEG-b-poly{N-[N-(2-aminoethyl)-2-aminoethyl]aspartamide}.
