Figure 5.

Effect of insulators on copy-number dependent expression. To check for a potential copy-number dependent effect after 30 days of cell expansion the mean fluorescent channel (MFC) values (y-axis) of the clones that contain uninsulated or insulated vectors were plotted with the number of integrated vectors (x-axis). The analysis were performed in clones bearing single or multiple integrated events with a minimum of six clones for each group. Linear regression analysis was used for statistical evaluation. VCN, vector copy number.