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Onset of OTC activity following vector delivery. Adult male Spfash mice were injected intraperitoneally with 1.5 × 1011 vg, and OTC enzyme activity determined in liver harvested at 1, 2, 3, 5, 7, and 14 days postinjection (n = 3–5 mice per time-point). Upper and lower horizontal lines represent liver OTC activity levels in wild-type and Spfash, respectively. Error bars represent mean ± SEM. Spfash, OTC-deficient; wt, wild-type; OTC, ornithine transcarbamylase; vg, vector genome.
