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. 2009 Jun 16;17(9):1537–1547. doi: 10.1038/mt.2009.134

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Copyright 2009, The American Society of Gene & Cell Therapy

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Locus-specific qPCR analysis of selected clones in γ-retroviral LSK and STHSC progeny. (a–c) Locus-specific qPCR analysis of selected clones in individual recipients of LSK cells. Relative quantification of a target gene amplicon was estimated in comparison to amplicon at early time points, corresponding week 5 for (a) LSK#11, (b) LSK#12, and week 11 for (c) LSK#15. (d) Locus-specific qPCR analysis of selected clones in recipient ST#32. Relative quantification of Evi1 amplicon was estimated in comparison to amplicon at time point 5 weeks after BMT; for Smad3, Edem2, Mrpl1 amplicons in comparison to 11 weeks. Each analysis was performed in triplicate. Error bars indicate standard deviations. BMT, bone marrow transplantation; LSK, lineage marker negative, Sca1⁺, and c-Kit⁺; qPCR, quantitative PCR; STHSC, short-term repopulating hematopoietic stem cell.