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. 2009 Jul 7;17(9):1594–1604. doi: 10.1038/mt.2009.145

Figure 6.

Figure 6

Activation of adenylate cyclase promotes differentiation of hematopoietic stem cells (HSCs) to “retinal pigment epithelium–like” cells. (a) Mouse Sca+ lin HSCs were treated for 3 days with forskolin (10 µmol/l) and rolipram (1 µmol/l) to stimulate adenylate cyclase activity. HSCs exhibited pigmentation after such treatment. These same cells were fixed and stained with primary antibody directed to CRALBP and then exposed to fluorescently labeled secondary antibody. (b) Image from c overlain on the image from a to show the colocalization of intense CRALBP staining with the pigmented cells. (c) CRALBP reactive cells in red and cell nuclei stained in blue with 4′,6-diamidino-2-phenylindole (DAPI) (d shows DAPI only channel). Many of the cells outside of the clumped pigmented cells also expressed CRALBP (e,f), suggesting partial differentiation. Bar = 50 µm.