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. 2003 Nov 14;100(24):13952–13957. doi: 10.1073/pnas.2335190100

Fig. 5.

Fig. 5.

NO exogenously applied by an NO donor as well as Zn2+ increase constitutive MT-1 and MT-2 mRNA expression. Wt MAEC were cultured for 6 h in the absence (lanes 1 and 4) or presence (lane 2) of 200 μM of the NO donor DETA/NO or of decomposed DETA/NO (DETA/NO–NO) (lane 3), or of 100 μM Zn2+ (lane 5). Total cellular RNA was isolated, and MT-1-, MT-2-, and GAPDH-specific RT-PCR were performed. NO derived from DETA/NO as well as Zn2+ increase constitutive MT-1 and MT-2 mRNA expression. Values are mean ± SD of three independent experiments. *, P < 0.02 as compared with lane 2.