Figure 1.

Biodistribution of transduced cells. (a) Mice (2–3 per group) were injected either subcutaneously (s.c.) or intravenously (i.v.) with phosphate buffered saline or 10⁸ IU GFP LV. At different time points, cells from spleen and lymph nodes (inguinal and popliteal) were collected, pooled, separated with anti-CD11c magnetic microbeads and stained with antibodies for flow cytometric analysis. The percentage of GFP⁺ DCs, macrophages, B cells and T cells was determined in (b) the lymph nodes and spleen 2 months after s.c. or i.v. injection, and (c) in lymph nodes 5 days after s.c. injection. DC, dendritic cell; GFP, green fluorescence protein; LV, lentiviral vector.