Figure 3.

BrdU incorporation by transduced cells. (a) A LV-encoding Thy1.1 was used to track transduced cells by antibody staining and flow cytometry. Mice (n = 3–5 per group) were injected intravenously with phosphate buffered saline or Thy1.1 LV in the tail vein. Two days later, they received 200 µg of BrdU intraperitoneally followed by provision of BrdU 0.8 mg/ml in the drinking water from days 2 to 12. The experiment was repeated but BrdU was started at day 20 and given up to day 30 after injection of the LV. Incorporation of BrdU was analyzed by flow cytometry in different subpopulations of CD11c⁺ and CD11c⁻ splenocytes. (b) Similar results were obtained for both periods of administration, representative plots are shown. BrdU, bromodeoxyuridine; DC, dendritic cell; GFP, green fluorescence protein; LV, lentiviral vector; MHC II, major histocompatibility complex 2; pDC, plasmacytoid dendritic cell.