. Author manuscript; available in PMC: 2010 Mar 9.

Published in final edited form as: J Immunol Methods. 2008 Nov 25;341(1-2):97–105. doi: 10.1016/j.jim.2008.11.001

Table 3.

Response surface results: time (in hours) and MOI that maximizes the predicted value of cytokine secretion for both live and inactive vaccinia virus

Cytokine	Virus	Predicted value^a	Standard error	Optimal time	Optimal MOI	Range of time ^b	Range of MOI ^b
IL-2	Live	5.67	1.21	11	5	8–24	4–5
IL-2	Inactive	5.96	1.64	16	5	12–36	3.5–5
IL-4	Live	1.42	0.99	192	5	145–192	0.05–5
IL-4	Inactive	2.01	1.30	168	0.05	96–192	0.05
IL-6	Live	nd ^c	nd ^c	nd ^c	nd ^c	nd ^c	nd ^c
IL-6	Inactive	22.98	3.65	192	5	192	3–5
IL-10	Live	3.10	1.61	192	0.05	156–192	0.05
IL-10	Inactive	3.88	1.69	192	0.05	175–192	0.05
IL-12p40	Live	−0.41	0.69	96	0.05	96	0.05
IL-12p40	Inactive	0.26	0.67	24	0.05	8–32	0.05
IL-12p70	Live	0.99	0.51	23.5	0.2	24	0.2–0.5
IL-12p70	Inactive	1.88	0.78	22	0.7	16–36	0.2–3
IL-18	Live	1.69	0.63	96	0.05	96	0.05
IL-18	Inactive	1.74	0.61	24	0.05	24	0.05 or 5.0
TNF-α	Live	5.32	1.56	17.5	0.5	8–42	0.2–1.0
TNF-α	Inactive	7.21	2.41	25	0.4	12–65	0.2–1.0
IL-1β	Live	4.59	1.26	96	1.3	10–96	0.5–5
IL-1β	Inactive	8.50	2.35	19	0.7	8–45	0.3–1.8
IFN-α	Live	11.76	2.27	96	0.2	80–96	0.05–0.8
IFN-α	Inactive	24.71	3.48	96	0.05	96	0.05
IFN-β	Live	1.75	0.54	14.5	5	12–24	1.5–5
IFN-β	Inactive	2.30	0.49	30.5	5	24–36	3–5
IFN-γ	Live	4.51	1.60	96	0.05	60–96	0.05
IFN-γ	Inactive	4.11	1.49	96	0.05	96	0.05

Maximum predicted value of cytokine secretion, defined as the difference between the mean of the stimulated cells and the mean of the unstimulated cells, divided by their common standard error.

Approximate range of time and MOI values yielding secretion levels considered statistically equivalent to the maximum predicted value.

Not determined.