Skip to main content
. Author manuscript; available in PMC: 2010 Mar 9.
Published in final edited form as: Blood. 2004 Aug 24;105(1):153–160. doi: 10.1182/blood-2004-03-0990

Figure 2. Effect of varying doses of cyclodextrin treatment on cholesterol depletion and TF-VIIa activity in fibroblasts.

Figure 2

Monolayers of WI-38 cells were treated with varying doses (1 to 10 mM) of mβCD (A–B) for 45 minutes. At the end of 45 minutes, cells were washed and used for determining cholesterol content (A) or cell surface TF-VIIa activity by adding factor VIIa (10 nM) and factor X (175 nM) to the monolayers (B). (n = 4 to 6, mean ± SE). * denotes significantly differs (P < .05) from the control (untreated cells). Panel C depicts the time course of factor X activation by TF-VIIa in control and cholesterol-depleted cells (10 mM mβCD treatment for 45 minutes). Two different concentrations of factor X were used, 175 nM (circles) and 1 µM (squares). Filled symbols represent control cells, and open symbols represent mβCD-treated cells.