Fig. 1.

Characterization of a new mutant strain 1C12. (A) Western analyses of axonemes revealed that RS in 1C12 axonemes lacked RSP6 only. Each protein was revealed by antibodies raised against individual molecules. Anti-RSP6 antibody cross-reacted with RSP4 and hence RSP4 band appeared weaker than RSP6. In this particular preparation, the spokehead proteins, RSP 1, 9 and 10 and the stalk chaperone HSP40 (RSP16) were less abundant in the 1C12 axonemes than those in the wild type control. This difference was not evident in most preparations. (B) Transmission electron microscopy of axoneme cross sections. Compared to WT, no obvious morphological defect can be found in every RS in the 1C12 axoneme. The samples were prepared from the early stationary phase liquid culture