Figure 2.

A59 replication is inhibited in response to IFN-β in BMM and MEF. Primary cells or cell lines were treated with indicated concentrations of IFN-β for 16 hours prior to infection with MHV-A59 at MOI=10. A59 was incubated with cells at 370 °C for 1h hour after which virus was removed and cells washed three times in excess of PBS before adding fresh media and returning cells to 37 °C incubation. (A,B) Total RNA from infected cells was isolated at several time intervals post infection and analyzed by qRT-PCR for viral mRNA7. Values are normalized to β-actin and expressed as fold over mock infected cells. (C,D) Supernatants were collected from infected 293T cells transiently expressing MHV-R (CEACAM1a) or L2 cells at times indicated and viral titers were determined by plaque assay in L2 cells. Data are representative of at least two independent experiments.