Fig. 3.

Very early rescue of UV irradiation via tipping results in embryos with normal LR asymmetry. (A) One-cell embryos were UV irradiated at either 0.2NT or 0.4NT and immediately pipetted into an agarose grid. The grid (depicted by a yellow rectangle) was then tipped at 0, 20, 30, 45 or 90 degrees for at least one cell division. (B) Statistical comparison of all embryos irradiated at 0.4NT indicates that late rescue of UV irradiation (via XSiamois or LiCl injections) produces significantly more heterotaxia than the randomizing effects of the UV irradiation itself (as indicated from the tipping rescue). Different letters indicate groups that are significantly different from each other (P<0.01). Sample sizes are indicated on the bars of the graph.