Fig. 1.
Genetic allatectomy and its effect on puparial size. (A)The ring gland of control CyO, UAS-grim (above) and allatectomized (CAX) Aug21>grim (below) wandering larvae showing the loss of the corpora allata (CA) in the latter. a, aorta; PG, prothoracic gland. Scale bar: 25 μm. (B,C) Sample puparia (B) and the pupal weights (C) of CyO, UAS-grim (control) and Aug21>grim (allatectomized) larvae raised on normal diet containing 1% ethanol or 1 ppm pyriproxyfen (JHM) in 1% ethanol. Bars represent the average ± s.d. for 50-60 puparia (one day after wandering). Different letters denote average weights that are significantly different (P≤0.0001, using one-way ANOVA and Tukey-Kramer HSD comparison of means).