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. 2010 Apr 1;137(7):1117–1126. doi: 10.1242/dev.037218

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Fig. 3. — Precocious appearance of the ecdysone receptor EcR-B1 in the optic lobes (OL) of Aug21>grim allatectomized (CAX) prepupae and its suppression by feeding larvae on a diet containing 1 ppm pyriproxyfen (JHM). (A-E) OL confocal sections at different times (X hours; hr) APF of (A,D) CyO, UAS-grim (control) prepupae, (B) CAX prepupae after feeding on normal diet, (C) CAX prepupa after feeding on the JHM diet, and (E) Met²⁷ prepupae. Arrows indicate EcR-B1-positive neurons. Insets in A-C show endogenous EcR-B1 in the mushroom body neurons. (D,E) Deadpan-positive neuroblasts (green); EcR-B1 neurons (red). cp, ‘central plug’ neurons produced by the neuroblasts of the inner proliferation zone (ipz); la, lamina; m, medulla; opz, outer proliferation zone. Scale bar: 25 μm.