Fig. 2.

LR binds to both αGMR and βGMR. (A) The 293T cells were transfected with LR (5 μg) or αGMR (5 μg) or cotransfected with LR (5 μg) and αGMR (5 μg). Total protein lysates and αGMR Ab S20 immunoprecipitated proteins were analyzed by SDS/PAGE followed by Western blotting analysis (WB) by using LR Ab anti-V5-HRP (Left) and αGMR Ab C18 (Right). (B) The 293T cells were transfected with βGMR (5 μg) or LR (5 μg) or cotransfected with βGMR (5 μg) and LR (5 μg). Total protein lysates and βGMR Ab S16-immunoprecipitated (IP) proteins were analyzed by SDS/PAGE followed by Western blotting by using LR Ab anti-V5-HRP (Lower) and βGMR Ab C20 (Upper).