Fig. 4.
Nur77-dependent induction of TRAIL and apoptosis in UC-5 cells. A, induction of TRAIL by DIM-C-pPhOCH3 and DIM-C-Ph. Cells were treated with Nur77-active C-DIMs (15 μM) for 24 h, and whole-cell lysates were analyzed for TRAIL or β-tubulin. B and C, RNA interference with iNur77. UC-5 cells were transfected with iScr (nonspecific) or iNur77 and treated with DMSO or 15 μM DIM-C-pPhOCH3, and whole-cell lysates were analyzed by Western blot analysis for Nur77, PARP cleavage, TRAIL, and β-tubulin proteins. Columns, mean of three replicate experiments for each treatment group; error bar, S.D. Protein levels were normalized to β-tubulin. B, ∗, P < 0.05, significant inhibition of Nur77 expression. C, ∗, significant induction of TRAIL and PARP cleavage; ∗∗, decreased expression of these proteins by iNur77.