Fig. 5.

Induction of gene expression by DIM-C-pPhOCH₃. A and B, induction of NAG-1, p8, CSE, and sestrin-2 in UC-5 cells. Cells were treated with DMSO or 15 μM DIM-C-pPhOCH₃ for 6 h, and NAG-1, p8, CSE, sestrin-2, and GAPDH (reference mRNA) were determined by RT-PCR. Induction responses were determined in three separate experiments. ∗, P < 0.05, significantly induced mRNA expression. C, effects of iNur77 on induced gene expression. UC-5 cells were transfected with iScr or iNur77, treated with DMSO or 15 μM DIM-C-pPhOCH₃ for 6 h, and analyzed by real-time PCR. Columns, mean of three replicate determinations for each treatment group; error bars, S.D. ∗, P < 0.05, significant induction by DIM-C-pPhOCH₃; #, significant decrease after transfection with iNur77.