Fig. 5.

Time course examining the effects of persistent CP55,940 treatment on heteromer (D_2L-CB₁-Venus) and homomer (D_2L-D_2L-Cerulean) formation. CAD cells were transiently transfected with CB₁-VN, D_2L-CC, and D_2L-CN followed by quantitative image analyses of the Venus/Cerulean ratios for the membrane and intracellular compartments. A, cells were incubated with 10 μM CP55,940 for 10, 20, or 30 h before image analysis. Data represents the average median Venus-to-Cerulean ratio values normalized to percentage of vehicle treatment (±S.E.M.) in four independent experiments. B, images from 20-h time point depicting the effects of CP55,940 on Venus and Cerulean signals.