Fig. 10.
Increase in NMDA receptor channel open probability by ATX. A, cell-attached patch recording from DIV-1 cerebrocortical neurons. NMDA receptor unitary currents were evoked by 100 μM NMDA and 100 μM glycine in the patch pipette (pipette potential = +60 mV, filtered at 5 kHz for representation, digitized at 40 kHz). Enhancement of NMDA receptor activity by bath application of 100 nM ATX. B, bath application of 100 nM ATX increased NMDA receptor channel Po, but not the mean open time (MOT) (n = 6, *, p < 0.05, paired t test). C, pooled dwell-time histograms were fitted using Channelab. The open-time histogram was fitted by the sum of three Gaussian components, and the shut-time histogram was fitted by the sum of five Gaussian components. The time constants and area are described under Results.