Photomicrographs of glomeruli stained with PAS (original magnification, 200×) at 50 weeks of age. Vehicle-treated OLETF rats show mesangial expansion accompanied by an accumulation of extracellular matrix and capillary wall thickening. Quantitative analyses confirm that the glomerular PAS-positive area in vehicle-treated OLETF rats is significantly larger than that in LETO rats. In OLETF rats, treatment with telmisartan, eplerenone, or their combination significantly decreases the PAS-positive area in glomeruli. However, there are no significant differences in glomerular PAS-positive area among telmisartan-, eplerenone- or telmisartan + eplerenone-treated OLETF rats. In contrast, hydralazine does not affect the glomerular PAS-positive area. ∗, P < 0.05 versus LETO rats. †, P < 0.05; OLETF + vehicle versus OLETF + telmisartan, eplerenone, telmisartan + eplerenone or hydralazine.