a, relative expression of PLTP mRNA in human liver, primary hepatocytes,
HepG2, and Huh7 cells. Gene expression was analyzed by TaqMan real-time
polymerase chain reaction as described under Materials and
Methods. The relative expression is presented relative to the
expression level in liver. b, inhibition of apoB secretion by compound A and
MTP inhibitor CP-346086 in Huh7 cells. c, dose-dependent inhibition of apoB
secretion by compound A in HepG2 cells. HepG2 or Huh7 cells were treated
with the compounds for 24 h, and medium was collected for apoB and
transferrin measurement by ELISA. Data are the average of triplicates
± S.D.