Dose-dependent analysis of growth inhibition of MCF10 variants by MEK inhibitors
in 2D and 3D culture. A and C, cells were cultured in 96-well plates for 3 days
with the indicated concentrations of U0126 or CI-1040 and subjected to MTT assay.
B and D, cells were cultured for 9 days in 3D rBM overlay cultures with the
indicated concentrations of U0126 or CI-1040 and the size of structures measured.
The cell lines assayed were MCF10A, black open circles; MCF10.NeoT, magenta
triangles; MCF10.AT1, orange triangles; MCF10.DCIS, red diamonds; MCF10.H-Ras,
green circles; and MCF10.N-Ras, blue squares. Data shown are mean ±
S.E.M. (n = 3).