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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Matrix Biol. 2009 Jul 28;28(8):463–469. doi: 10.1016/j.matbio.2009.07.005

Fig. 1.

Fig. 1

Purification of TIMP-3 and N-TIMP-3. FLAG-tagged glycosylated human TIMP-3 and N-TIMP-3 were expressed by treating transfected HEK293 with 30 mM sodium chlorate in serum-free DMEM and purified from the conditioned medium by FLAG affinity chromatography and cation exchange chromatography. A, Silver-stained 10% polyacrylamide SDS-PAGE gel of TIMP-3 (100 ng) and N-TIMP-3 (100 ng). B, Immuoblot of 12% polyacrylamide SDS-PAGE gel of TIMP-3 (100 ng) and N-TIMP-3 (100 ng) stained using a polyclonal rabbit anti-TIMP-3 antibody.